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About
Colony Forming Cell Assays
Hematopoietic progenitors with lineage-restricted (less primitive) or multi-lineage (more primitive) potential form morphologically distinct colonies when grown in a suitable semi-solid medium containing appropriate growth factors and other supplements. To form the specific colony type that characterizes its potential, a progenitor cell must both proliferate and differentiate within the supportive matrix.
Colony-forming cell (CFC) assays therefore have great utility in the identification and enumeration of the different types of functional progenitors in hematopoietic tissues (e.g. bone marrow, cord blood, fetal liver) and manipulated cell populations (e.g. purified, expanded, frozen/thawed, drug treated, etc.).
For a variety of reasons, methylcellulose has become the matrix of choice for semi-solid media for CFC assays. Pre-screening and qualification of the various components of methylcellulose-based media allow for media products that are highly reproducible in their ability to support vigorous and robust growth of the various types of progenitors.
ColonyGEL™ meets the highest standards of methylcellulose-based media for hematopoietic colony-forming cell assays.
ColonyGEL™ combines uncompromising quality and performance with GREAT value
Features & Benefits
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Pre-screened and carefully selected components
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Reproducible and reliable - used in ReachBio’s own contract service labs for high value drug development applications
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Great optical clarity
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Manufactured in the USA in accordance with the
highest standards
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Meets or beats the performance of the current gold
standard CFC media
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Various optimized formulations
available
- with or without Epo
- standard or enhanced
cytokine content
- species-specific formulations -
each performance tested with primary progenitor
cells of the relevant species
- base medium also
available – allows addition of your own growth
factors for unique applications
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Suitable for identifying and enumerating:
- CFU-E
- BFU-E
- CFU-G
- CFU-M
- CFU-GM
- CFU-GEMM
- Pre-B (mouse only)
Additional Advantages
Plus!
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